hitrap sp hp 5ml (Thermo Fisher)
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hitrap sp hp 5ml
Hitrap Sp Hp 5ml, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hitrap+sp+hp+5ml/hitrap+sp+hp+5ml/pmc11912512-65-0-6
Average 90 stars, based on 1 article reviews
Hitrap Sp Hp 5ml, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hitrap+sp+hp+5ml/hitrap+sp+hp+5ml/pmc11912512-65-0-6
Average 90 stars, based on 1 article reviews
hitrap sp hp 5ml - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "Structural insights into HIV-2 CA lattice formation and FG-pocket binding revealed by single-particle cryo-EM"
Article Title: Structural insights into HIV-2 CA lattice formation and FG-pocket binding revealed by single-particle cryo-EM
Journal: Cell reports
doi: 10.1016/j.celrep.2025.115245
Figure Legend Snippet:
Techniques Used: Virus, Recombinant, Protease Inhibitor, Electron Microscopy, Liposomes, Software
Related Articles
other:Article Title: Structural insights into HIV-2 CA lattice formation and FG-pocket binding revealed by single-particle cryo-EM Article Snippet: Liposomes:Article Title: Structural insights into HIV-2 CA lattice formation and FG-pocket binding revealed by single-particle cryo-EM. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli BL21(DE3) Lucigen Cat#60401 Chemicals, peptides, and recombinant proteins Luria Broth Research Products International Cat#L24400 Terrific Broth Research Products International Cat#T5100–5000.0 Isopropyl b-D-1-thiogalactopyranoside (IPTG) American Bioanalytical Cat#AB00841-00010 HaltTM Protease Inhibitor Cocktail (100X) Thermo Scientific Cat#78430 Trizma Base Sigma Aldrich Cat#T1503 2-Mercaptoethanol (BME) Alfa Aesar Cat#J66742-0B Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) Thermo Scientific Cat#20490 Ammonium Sulfate Thermo Scientific Cat#J64419.36 4-(2-Hydroxyethyl)piperazine-1ethanesulfonic acid, N-(2-Hydroxyethyl) piperazine-N0-(2-ethanesulfonic acid) (HEPES) Sigma Aldrich Cat#391340 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1carboxypentyl)iminodiacetic acid)succinyl] nickel salt (DGS-NiNTA) Avanti Polar Lipids Cat#790404 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) Avanti Polar Lipids Cat#850375 Cholesterol Thermo Scientific Cat#A11470 Uranyl Acetate Electron Microscopy Services Cat#22400 NuPAGETM LDS Sample Buffer (4X) Invitrogen Cat#NP0008 NuPAGETM MES SDS Running Buffer (20X) Invitrogen Cat#NP0002 SimplyBlueTM SafeStain Thermo Scientific Cat#LC6060 Q5 High-Fidelity DNA Polymerase New England Biolabs Cat#M0491 Gibson Assembly Master Mix New England Biolabs Cat#E2611 Nup153 peptide (residues 1411–1425/ 1464-1475) GenScript N/A CPSF6 peptide (residues 313–327) GenScript N/A Recombinant GL-AN HIV-2 CA-GSSHHHHHH This paper N/A Recombinant NL4-3 HIV-1 CA-GSSHHHHHH This paper N/A Deposited data EM Map of HIV-2 CA Icosahedron Templated on Liposomes This paper EMD-45676 EM Map of HIV-2 CA Hexamer Templated on Liposomes This paper EMD-45758 EM Map of HIV-2 CA Pentamer Templated on Liposomes This paper EMD-45759 EM Map of HIV-2 CA Hexamer Templated on Liposomes with Nup153 Peptide Bound This paper EMD-45760 EM Map of HIV-2 CA Hexamer Templated on Liposomes with CPSF6 Peptide Bound This paper EMD-45761 (Continued on next page) 14 Cell Reports 44, 115245, February 25, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER EM Map of HIV-2 CA Pentamer Templated on Liposomes with Nup153 Peptide in Solution This paper EMD-45762 EM Map of HIV-2 CA Pentamer Templated on Liposomes with CPSF6 Peptide in Solution This paper EMD-45763 EM Map of HIV-1 CA Pentamer Templated on Liposomes This paper EMD-47600 Atomic Model of HIV-2 CA Icosahedron Templated on Liposomes This paper PDB: 9CLJ Atomic Model of HIV-2 CA Hexamer Templated on Liposomes This paper PDB: 9CNS Atomic Model of HIV-2 CA Pentamer Templated on Liposomes This paper PDB: 9CNT Atomic Model of HIV-2 CA Hexamer Templated on Liposomes with Nup153 Peptide Bound This paper PDB: 9CNU Atomic Model of HIV-2 CA Hexamer Templated on Liposomes with CPSF6 Peptide Bound This paper PDB: 9CNV Recombinant DNA pET11a GL-AN HIV-2 CA-GSSHHHHHH This paper GenBank: PQ189021 Software and algorithms SerialEM Mastronarde et al.89 https://bio3d.colorado.edu/SerialEM/ download.html CryoSPARC Punjani et al.90 https://guide.cryosparc.com/setup- configuration-and-management/how-todownload-install-and-configure Bayesian Motion Correction Zivanov et al.91 N/A Non-Uniform Refinement Punjani et al.93 N/A Coot Emsley et al.95 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Phenix Liebschner et al.97 https://phenix-online.org/download/ MolProbity Williams et al.98 N/A ChimeraX Goddard et al.99 https://www.cgl.ucsf.edu/chimerax/ download.html PRODIGY Xue et al.82 https://rascar.science.uu.nl/prodigy/ Other Ni-NTA Agarose Qiagen Cat#30230 HiTrap SP HP 5mL Cytiva Cat#17115101 Recombinant:Article Title: Structural insights into HIV-2 CA lattice formation and FG-pocket binding revealed by single-particle cryo-EM. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli BL21(DE3) Lucigen Cat#60401 Chemicals, peptides, and recombinant proteins Luria Broth Research Products International Cat#L24400 Terrific Broth Research Products International Cat#T5100–5000.0 Isopropyl b-D-1-thiogalactopyranoside (IPTG) American Bioanalytical Cat#AB00841-00010 HaltTM Protease Inhibitor Cocktail (100X) Thermo Scientific Cat#78430 Trizma Base Sigma Aldrich Cat#T1503 2-Mercaptoethanol (BME) Alfa Aesar Cat#J66742-0B Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) Thermo Scientific Cat#20490 Ammonium Sulfate Thermo Scientific Cat#J64419.36 4-(2-Hydroxyethyl)piperazine-1ethanesulfonic acid, N-(2-Hydroxyethyl) piperazine-N0-(2-ethanesulfonic acid) (HEPES) Sigma Aldrich Cat#391340 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1carboxypentyl)iminodiacetic acid)succinyl] nickel salt (DGS-NiNTA) Avanti Polar Lipids Cat#790404 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) Avanti Polar Lipids Cat#850375 Cholesterol Thermo Scientific Cat#A11470 Uranyl Acetate Electron Microscopy Services Cat#22400 NuPAGETM LDS Sample Buffer (4X) Invitrogen Cat#NP0008 NuPAGETM MES SDS Running Buffer (20X) Invitrogen Cat#NP0002 SimplyBlueTM SafeStain Thermo Scientific Cat#LC6060 Q5 High-Fidelity DNA Polymerase New England Biolabs Cat#M0491 Gibson Assembly Master Mix New England Biolabs Cat#E2611 Nup153 peptide (residues 1411–1425/ 1464-1475) GenScript N/A CPSF6 peptide (residues 313–327) GenScript N/A Recombinant GL-AN HIV-2 CA-GSSHHHHHH This paper N/A Recombinant NL4-3 HIV-1 CA-GSSHHHHHH This paper N/A Deposited data EM Map of HIV-2 CA Icosahedron Templated on Liposomes This paper EMD-45676 EM Map of HIV-2 CA Hexamer Templated on Liposomes This paper EMD-45758 EM Map of HIV-2 CA Pentamer Templated on Liposomes This paper EMD-45759 EM Map of HIV-2 CA Hexamer Templated on Liposomes with Nup153 Peptide Bound This paper EMD-45760 EM Map of HIV-2 CA Hexamer Templated on Liposomes with CPSF6 Peptide Bound This paper EMD-45761 (Continued on next page) 14 Cell Reports 44, 115245, February 25, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER EM Map of HIV-2 CA Pentamer Templated on Liposomes with Nup153 Peptide in Solution This paper EMD-45762 EM Map of HIV-2 CA Pentamer Templated on Liposomes with CPSF6 Peptide in Solution This paper EMD-45763 EM Map of HIV-1 CA Pentamer Templated on Liposomes This paper EMD-47600 Atomic Model of HIV-2 CA Icosahedron Templated on Liposomes This paper PDB: 9CLJ Atomic Model of HIV-2 CA Hexamer Templated on Liposomes This paper PDB: 9CNS Atomic Model of HIV-2 CA Pentamer Templated on Liposomes This paper PDB: 9CNT Atomic Model of HIV-2 CA Hexamer Templated on Liposomes with Nup153 Peptide Bound This paper PDB: 9CNU Atomic Model of HIV-2 CA Hexamer Templated on Liposomes with CPSF6 Peptide Bound This paper PDB: 9CNV Recombinant DNA pET11a GL-AN HIV-2 CA-GSSHHHHHH This paper GenBank: PQ189021 Software and algorithms SerialEM Mastronarde et al.89 https://bio3d.colorado.edu/SerialEM/ download.html CryoSPARC Punjani et al.90 https://guide.cryosparc.com/setup- configuration-and-management/how-todownload-install-and-configure Bayesian Motion Correction Zivanov et al.91 N/A Non-Uniform Refinement Punjani et al.93 N/A Coot Emsley et al.95 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Phenix Liebschner et al.97 https://phenix-online.org/download/ MolProbity Williams et al.98 N/A ChimeraX Goddard et al.99 https://www.cgl.ucsf.edu/chimerax/ download.html PRODIGY Xue et al.82 https://rascar.science.uu.nl/prodigy/ Other Ni-NTA Agarose Qiagen Cat#30230 HiTrap SP HP 5mL Cytiva Cat#17115101 Software:Article Title: Structural insights into HIV-2 CA lattice formation and FG-pocket binding revealed by single-particle cryo-EM. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli BL21(DE3) Lucigen Cat#60401 Chemicals, peptides, and recombinant proteins Luria Broth Research Products International Cat#L24400 Terrific Broth Research Products International Cat#T5100–5000.0 Isopropyl b-D-1-thiogalactopyranoside (IPTG) American Bioanalytical Cat#AB00841-00010 HaltTM Protease Inhibitor Cocktail (100X) Thermo Scientific Cat#78430 Trizma Base Sigma Aldrich Cat#T1503 2-Mercaptoethanol (BME) Alfa Aesar Cat#J66742-0B Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) Thermo Scientific Cat#20490 Ammonium Sulfate Thermo Scientific Cat#J64419.36 4-(2-Hydroxyethyl)piperazine-1ethanesulfonic acid, N-(2-Hydroxyethyl) piperazine-N0-(2-ethanesulfonic acid) (HEPES) Sigma Aldrich Cat#391340 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1carboxypentyl)iminodiacetic acid)succinyl] nickel salt (DGS-NiNTA) Avanti Polar Lipids Cat#790404 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) Avanti Polar Lipids Cat#850375 Cholesterol Thermo Scientific Cat#A11470 Uranyl Acetate Electron Microscopy Services Cat#22400 NuPAGETM LDS Sample Buffer (4X) Invitrogen Cat#NP0008 NuPAGETM MES SDS Running Buffer (20X) Invitrogen Cat#NP0002 SimplyBlueTM SafeStain Thermo Scientific Cat#LC6060 Q5 High-Fidelity DNA Polymerase New England Biolabs Cat#M0491 Gibson Assembly Master Mix New England Biolabs Cat#E2611 Nup153 peptide (residues 1411–1425/ 1464-1475) GenScript N/A CPSF6 peptide (residues 313–327) GenScript N/A Recombinant GL-AN HIV-2 CA-GSSHHHHHH This paper N/A Recombinant NL4-3 HIV-1 CA-GSSHHHHHH This paper N/A Deposited data EM Map of HIV-2 CA Icosahedron Templated on Liposomes This paper EMD-45676 EM Map of HIV-2 CA Hexamer Templated on Liposomes This paper EMD-45758 EM Map of HIV-2 CA Pentamer Templated on Liposomes This paper EMD-45759 EM Map of HIV-2 CA Hexamer Templated on Liposomes with Nup153 Peptide Bound This paper EMD-45760 EM Map of HIV-2 CA Hexamer Templated on Liposomes with CPSF6 Peptide Bound This paper EMD-45761 (Continued on next page) 14 Cell Reports 44, 115245, February 25, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER EM Map of HIV-2 CA Pentamer Templated on Liposomes with Nup153 Peptide in Solution This paper EMD-45762 EM Map of HIV-2 CA Pentamer Templated on Liposomes with CPSF6 Peptide in Solution This paper EMD-45763 EM Map of HIV-1 CA Pentamer Templated on Liposomes This paper EMD-47600 Atomic Model of HIV-2 CA Icosahedron Templated on Liposomes This paper PDB: 9CLJ Atomic Model of HIV-2 CA Hexamer Templated on Liposomes This paper PDB: 9CNS Atomic Model of HIV-2 CA Pentamer Templated on Liposomes This paper PDB: 9CNT Atomic Model of HIV-2 CA Hexamer Templated on Liposomes with Nup153 Peptide Bound This paper PDB: 9CNU Atomic Model of HIV-2 CA Hexamer Templated on Liposomes with CPSF6 Peptide Bound This paper PDB: 9CNV Recombinant DNA pET11a GL-AN HIV-2 CA-GSSHHHHHH This paper GenBank: PQ189021 Software and algorithms SerialEM Mastronarde et al.89 https://bio3d.colorado.edu/SerialEM/ download.html CryoSPARC Punjani et al.90 https://guide.cryosparc.com/setup- configuration-and-management/how-todownload-install-and-configure Bayesian Motion Correction Zivanov et al.91 N/A Non-Uniform Refinement Punjani et al.93 N/A Coot Emsley et al.95 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Phenix Liebschner et al.97 https://phenix-online.org/download/ MolProbity Williams et al.98 N/A ChimeraX Goddard et al.99 https://www.cgl.ucsf.edu/chimerax/ download.html PRODIGY Xue et al.82 https://rascar.science.uu.nl/prodigy/ Other Ni-NTA Agarose Qiagen Cat#30230 HiTrap SP HP 5mL Cytiva Cat#17115101 SDS Page:Article Title: Structural insights into HIV-2 CA lattice formation and FG-pocket binding revealed by single-particle cryo-EM. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains E. coli BL21(DE3) Lucigen Cat#60401 Chemicals, peptides, and recombinant proteins Luria Broth Research Products International Cat#L24400 Terrific Broth Research Products International Cat#T5100–5000.0 Isopropyl b-D-1-thiogalactopyranoside (IPTG) American Bioanalytical Cat#AB00841-00010 HaltTM Protease Inhibitor Cocktail (100X) Thermo Scientific Cat#78430 Trizma Base Sigma Aldrich Cat#T1503 2-Mercaptoethanol (BME) Alfa Aesar Cat#J66742-0B Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) Thermo Scientific Cat#20490 Ammonium Sulfate Thermo Scientific Cat#J64419.36 4-(2-Hydroxyethyl)piperazine-1ethanesulfonic acid, N-(2-Hydroxyethyl) piperazine-N0-(2-ethanesulfonic acid) (HEPES) Sigma Aldrich Cat#391340 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1carboxypentyl)iminodiacetic acid)succinyl] nickel salt (DGS-NiNTA) Avanti Polar Lipids Cat#790404 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) Avanti Polar Lipids Cat#850375 Cholesterol Thermo Scientific Cat#A11470 Uranyl Acetate Electron Microscopy Services Cat#22400 NuPAGETM LDS Sample Buffer (4X) Invitrogen Cat#NP0008 NuPAGETM MES SDS Running Buffer (20X) Invitrogen Cat#NP0002 SimplyBlueTM SafeStain Thermo Scientific Cat#LC6060 Q5 High-Fidelity DNA Polymerase New England Biolabs Cat#M0491 Gibson Assembly Master Mix New England Biolabs Cat#E2611 Nup153 peptide (residues 1411–1425/ 1464-1475) GenScript N/A CPSF6 peptide (residues 313–327) GenScript N/A Recombinant GL-AN HIV-2 CA-GSSHHHHHH This paper N/A Recombinant NL4-3 HIV-1 CA-GSSHHHHHH This paper N/A Deposited data EM Map of HIV-2 CA Icosahedron Templated on Liposomes This paper EMD-45676 EM Map of HIV-2 CA Hexamer Templated on Liposomes This paper EMD-45758 EM Map of HIV-2 CA Pentamer Templated on Liposomes This paper EMD-45759 EM Map of HIV-2 CA Hexamer Templated on Liposomes with Nup153 Peptide Bound This paper EMD-45760 EM Map of HIV-2 CA Hexamer Templated on Liposomes with CPSF6 Peptide Bound This paper EMD-45761 (Continued on next page) 14 Cell Reports 44, 115245, February 25, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER EM Map of HIV-2 CA Pentamer Templated on Liposomes with Nup153 Peptide in Solution This paper EMD-45762 EM Map of HIV-2 CA Pentamer Templated on Liposomes with CPSF6 Peptide in Solution This paper EMD-45763 EM Map of HIV-1 CA Pentamer Templated on Liposomes This paper EMD-47600 Atomic Model of HIV-2 CA Icosahedron Templated on Liposomes This paper PDB: 9CLJ Atomic Model of HIV-2 CA Hexamer Templated on Liposomes This paper PDB: 9CNS Atomic Model of HIV-2 CA Pentamer Templated on Liposomes This paper PDB: 9CNT Atomic Model of HIV-2 CA Hexamer Templated on Liposomes with Nup153 Peptide Bound This paper PDB: 9CNU Atomic Model of HIV-2 CA Hexamer Templated on Liposomes with CPSF6 Peptide Bound This paper PDB: 9CNV Recombinant DNA pET11a GL-AN HIV-2 CA-GSSHHHHHH This paper GenBank: PQ189021 Software and algorithms SerialEM Mastronarde et al.89 https://bio3d.colorado.edu/SerialEM/ download.html CryoSPARC Punjani et al.90 https://guide.cryosparc.com/setup- configuration-and-management/how-todownload-install-and-configure Bayesian Motion Correction Zivanov et al.91 N/A Non-Uniform Refinement Punjani et al.93 N/A Coot Emsley et al.95 https://www2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Phenix Liebschner et al.97 https://phenix-online.org/download/ MolProbity Williams et al.98 N/A ChimeraX Goddard et al.99 https://www.cgl.ucsf.edu/chimerax/ download.html PRODIGY Xue et al.82 https://rascar.science.uu.nl/prodigy/ Other Ni-NTA Agarose Qiagen Cat#30230 HiTrap SP HP 5mL Cytiva Cat#17115101 |
